Squash hybrid sv0201yl and parents thereof

ABSTRACT

The invention provides seed and plants of squash hybrid SV0201YL and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of squash hybrid SV0201YL and the parent lines thereof, and to methods for producing a squash plant produced by crossing such plants with themselves or with another squash plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the priority of U.S. Provisional Appl. Ser. No.61/732,204, filed Nov. 30, 2012, the entire disclosure of which isincorporated herein by reference.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of squash hybrid SV0201YL and theinbred squash lines LEB-EH-08-877 and ZGN-130-1091.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,resistance to insects or disease, tolerance to environmental stress, andnutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a squash plant of thehybrid designated SV0201YL, the squash line LEB-EH-08-877 or squash lineZGN-130-1091. Also provided are squash plants having all thephysiological and morphological characteristics of such a plant. Partsof these squash plants are also provided, for example, including pollen,an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of squash hybrid SV0201YLand/or squash lines LEB-EH-08-877 and ZGN-130-1091 comprising an addedheritable trait is provided. The heritable trait may comprise a geneticlocus that is, for example, a dominant or recessive allele. In oneembodiment of the invention, a plant of squash hybrid SV0201YL and/orsquash lines LEB-EH-08-877 and ZGN-130-1091 is defined as comprising asingle locus conversion. In specific embodiments of the invention, anadded genetic locus confers one or more traits such as, for example,herbicide tolerance, insect resistance, disease resistance, and modifiedcarbohydrate metabolism. In further embodiments, the trait may beconferred by a naturally occurring gene introduced into the genome of aline by backcrossing, a natural or induced mutation, or a transgeneintroduced through genetic transformation techniques into the plant or aprogenitor of any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of squash hybrid SV0201YL and/orsquash lines LEB-EH-08-877 and ZGN-130-1091. The squash seed of theinvention may be provided as an essentially homogeneous population ofsquash seed of squash hybrid SV0201YL and/or squash lines LEB-EH-08-877and ZGN-130-1091. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, seedof hybrid SV0201YL and/or squash lines LEB-EH-08-877 and ZGN-130-1091may be defined as forming at least about 97% of the total seed,including at least about 98%, 99% or more of the seed. The seedpopulation may be separately grown to provide an essentially homogeneouspopulation of squash plants designated SV0201YL and/or squash linesLEB-EH-08-877 and ZGN-130-1091.

In yet another aspect of the invention, a tissue culture of regenerablecells of a squash plant of hybrid SV0201YL and/or squash linesLEB-EH-08-877 and ZGN-130-1091 is provided. The tissue culture willpreferably be capable of regenerating squash plants capable ofexpressing all of the physiological and morphological characteristics ofthe starting plant, and of regenerating plants having substantially thesame genotype as the starting plant. Examples of some of thephysiological and morphological characteristics of the hybrid SV0201YLand/or squash lines LEB-EH-08-877 and ZGN-130-1091 include those traitsset forth in the tables herein. The regenerable cells in such tissuecultures may be derived, for example, from embryos, meristems,cotyledons, pollen, leaves, anthers, roots, root tips, pistils, flowers,seed and stalks. Still further, the present invention provides squashplants regenerated from a tissue culture of the invention, the plantshaving all the physiological and morphological characteristics of hybridSV0201YL and/or squash lines LEB-EH-08-877 and ZGN-130-1091.

In still yet another aspect of the invention, processes are provided forproducing squash seeds, plants and fruit, which processes generallycomprise crossing a first parent squash plant with a second parentsquash plant, wherein at least one of the first or second parent squashplants is a plant of squash line LEB-EH-08-877 or squash lineZGN-130-1091. These processes may be further exemplified as processesfor preparing hybrid squash seed or plants, wherein a first squash plantis crossed with a second squash plant of a different, distinct genotypeto provide a hybrid that has, as one of its parents, a plant of squashline LEB-EH-08-877 or squash line ZGN-130-1091. In these processes,crossing will result in the production of seed. The seed productionoccurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent squash plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent squash plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent squash plants. Yet another step comprisesharvesting the seeds from at least one of the parent squash plants. Theharvested seed can be grown to produce a squash plant or hybrid squashplant.

The present invention also provides the squash seeds and plants producedby a process that comprises crossing a first parent squash plant with asecond parent squash plant, wherein at least one of the first or secondparent squash plants is a plant of squash hybrid SV0201YL and/or squashlines LEB-EH-08-877 and ZGN-130-1091. In one embodiment of theinvention, squash seed and plants produced by the process are firstgeneration (F₁) hybrid squash seed and plants produced by crossing aplant in accordance with the invention with another, distinct plant. Thepresent invention further contemplates plant parts of such an F₁ hybridsquash plant, and methods of use thereof. Therefore, certain exemplaryembodiments of the invention provide an F₁ hybrid squash plant and seedthereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SV0201YL and/or squash linesLEB-EH-08-877 and ZGN-130-1091, the method comprising the steps of: (a)preparing a progeny plant derived from hybrid SV0201YL and/or squashlines LEB-EH-08-877 and ZGN-130-1091, wherein said preparing comprisescrossing a plant of the hybrid SV0201YL and/or squash linesLEB-EH-08-877 and ZGN-130-1091 with a second plant; and (b) crossing theprogeny plant with itself or a second plant to produce a seed of aprogeny plant of a subsequent generation. In further embodiments, themethod may additionally comprise: (c) growing a progeny plant of asubsequent generation from said seed of a progeny plant of a subsequentgeneration and crossing the progeny plant of a subsequent generationwith itself or a second plant; and repeating the steps for an additional3-10 generations to produce a plant derived from hybrid SV0201YL and/orsquash lines LEB-EH-08-877 and ZGN-130-1091. The plant derived fromhybrid SV0201YL and/or squash lines LEB-EH-08-877 and ZGN-130-1091 maybe an inbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom hybrid SV0201YL and/or squash lines LEB-EH-08-877 and ZGN-130-1091is obtained which possesses some of the desirable traits of theline/hybrid as well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of squashhybrid SV0201YL and/or squash lines LEB-EH-08-877 and ZGN-130-1091,wherein the plant has been cultivated to maturity, and (b) collecting atleast one squash from the plant.

In still yet another aspect of the invention, the genetic complement ofsquash hybrid SV0201YL and/or squash lines LEB-EH-08-877 andZGN-130-1091 is provided. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a squash plant,or a cell or tissue of that plant. A genetic complement thus representsthe genetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make up of a hybrid cell, tissue orplant. The invention thus provides squash plant cells that have agenetic complement in accordance with the squash plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SV0201YL and/or squash lines LEB-EH-08-877and ZGN-130-1091 could be identified by any of the many well knowntechniques such as, for example, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by squash plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a squash plant of the invention with a haploid geneticcomplement of a second squash plant, preferably, another, distinctsquash plant. In another aspect, the present invention provides a squashplant regenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of squash hybrid SV0201YL, squash lineLEB-EH-08-877 and squash line ZGN-130-1091.

Squash hybrid SV0201YL, also known as LEBEHH0201, is a longLebanese-type squash, similar to the market class known best by thecommercial products Eskenderany, Otto, Revera, and Marzouka. SV0201YLhas uniform cylindrical fruit, somewhat darker in color than some otherhybrids, a very vigorous growth habit, and resistance for at least 3important viral diseases: ZYMV, WMV, and SLCV.

A. ORIGIN AND BREEDING HISTORY OF SQUASH HYBRID SV0201YL

The parents of hybrid SV0201YL are LEB-EH-08-877 and ZGN-130-1091. Theseparents were created as follows:

Squash line LEB-EH-08-877 was developed by 10 generations of inbreedingfollowing a cross of the proprietary inbred line LEB-47-5004 andLEB-47-103.

Selection of inbred line LEB-EH-08-877 required the following germplasmsources: (1 and 2) distinct selections from Turkish landraces of squashknown locally as “beyaz kabak”, (3) a selection from the common landrace“Small Green Algerian,” and (4) the diverse F1 family known as“ZUCX85-187-3” distributed by Henry Munger of Cornell University in1986.

LEB-EH-08-877 traces lineage back to ZUCX85-187-3. In 1986 an individualplant selection from the diverse F1 family known as ZUCX85-187-3 wascrossed to the landrace “Small Green Algerian.” An individual plant fromthis family was selected and used as a male parent in a cross with aselection from the “Small Green Algerian” landrace. The F1 seed fromthis cross was self pollinated, and between 1987 and 1992 was advancedto the F8 generation at breeding nurseries in New Jersey and Florida,guided by progeny testing results with ZYMV and WMV screening. In 1994an F7 selection from this lineage was artificially inoculated atWoodland Calif. with ZYMV, and the family was observed to be uniformlyresistant. A surviving plant from that screen was used as the femaleparent in a cross to a selection of the landrace “beyaz kabak”, and theresulting F1 hybrid was self pollinated at a greenhouse in Woodland,Calif. In 1995 the F2 generation was inoculated with ZYMV, and survivorswere selected for conformity to the Lebanese ideotype (white fruitcolor, thick leaves, strong canopy, high vigor). The same screen wasrepeated on the F3 generation in 1996. The F4 and F5 generations wereselected and self pollinated based only on phenotypic conformity to theLebanese ideotype. In 1997 four individual plants from the F6 generationshowing phenotypic uniformity were selected and bulked to create theproprietary inbred line LEB-47-5004.

In 1998 the proprietary inbred LEB-47-5004 was shared with the squashbreeding program of the legacy company of Royal Sluis in Nimes, France.LEB-47-5004 was then hybridized to a different selection of the landraceknown as “beyaz kabak,” and between 1998 and 2006 ten generations ofselection and self pollination with progeny testing for resistance toZYMV and WMV were performed to recombine the resistance to potyvirusesfound LEB-47-5004 with a fruit shape and color that better adheres tothe Lebanese ideotype. In 2008 the self pollinated progeny of a singleF11 generation plant was designated as LEB-EH-08-877, and all subsequenttesting and hybrids were developed from the progeny of that source.

Squash line ZGN-130-1091 was developed by a selection strategy involvingfive primary components: (1) ZGN-130-1003, a proprietary breeding linecontributing fruit quality characteristics and some potyvirus resistancethat is itself derived directly from the commercial hybrid “Tigress”,(2) ZGY-46-2604, the original gray zucchini source of resistance toSLCV, which was distributed by Sergio Garza from the Universidad deSonora in Hermosillo, Sonora, Mexico, in 1998, (3) G710, a proprietarybreeding line contributing fruit quality characteristics, derived fromthe commercial hybrid “Black Beauty”, (4) HP13HMW/HP134*1, a breedingline contributing high resistance to ZYMV, WMV-2, and PRSV, and (5) theproprietary inbred ZGN-130-1020, a vigorous green zucchini parent linewith resistance to ZYMV and WMV-2. ZGN-130-1091 is derived fromgermplasm with high levels of resistance to SLCV, an unexpected resultof the combination of low levels of resistance from ZGY 46-2604 and theSLCV susceptible breeding line HP13HMW/HP134*1. ZGN-130-1091 is derivedfrom germplasm with high levels of resistance to SLCV, an unexpectedresult of the combination of low levels of resistance from ZGY 46-2604and the SLCV susceptible breeding line HP13HMW/HP134*1, which is thesubject of different intellectual property filings, U.S. applicationSer. No. 10/761,877 and U.S. Pat. No. 8,288,617 (from U.S. patentapplication Ser. No. 12/197,908).

ZGY-46-2604 was crossed to a series of breeding lines, including G710 inorder to develop breeding populations with a combination of SLCVresistance and acceptable fruit types. The resulting F1 progeny of thiscross was backcrossed to G710, and the BC1F2, BC1F3, and BC1F4generations were selected for fruit and plant characteristics withoutregard to SLCV virus resistance in Tifton, Ga.

The self-pollinated BC1F5 progeny selections were included in a fieldtrial in the Rio Grande Valley of Texas in late summer 2000 (a locationand date chosen for its history of SLCV epidemics). Heavy SLCV pressureand high levels of potyvirus infestations in the same field allowed forthe selections of breeding lines with SLCV and potyvirus resistance.Nineteen selections (or remnant seed in the case of failed pollinations)were chosen to be planted in the greenhouse at Woodland, Calif. inFebruary, 2001 and inoculated with SLCV. Four BC1F5 and BC1F6 familieswere selected for a high level of resistance to SLCV and for fruitquality characteristics.

The selected families were crossed to a series of thirty-one breedinglines in the spring of 2001. During the summer of 2001, these breedinglines were evaluated for virus resistance through individual andcocktail screens of potyviruses and for fruit quality characteristics inWoodland, Calif. Ten hybrids were selected, and these ten hybrids weresubsequently evaluated for resistance to SLCV in a nursery planted inHermosillo, Mexico in the latter part of 2001. The hybrid(ZGN-130-1003/(ZGY-46-2604/G710*1)) was selected for development of newgermplasm. This hybrid was planted in the greenhouse at Woodland, Calif.in February of 2002, and was crossed to proprietary inbred lineHP13HMW/HP134*1.

The resulting F1 population, known as(ZGN-130-1003/(ZGY-46-2604/G710*1F6))/(HP13HMW/HP134*1), was inoculatedwith a cocktail of ZYMV, WMV-2, and PRSV, and survivors were moved tothe field in Woodland, Calif. in the summer of 2002. This survivorpopulation and all its progeny were named TAW. Thirty-seven individualswere selected from the TAW population based on potyvirus resistance andfruit and plant characteristics. The F2 generations from these thirtyseven selections were sown in the greenhouse in Woodland, Calif. inJanuary 2003, and inoculated with ZYMV, WMV-2, PRSV, and SLCV.

The high SLCV resistance described was first observed in this early 2003screen, where F2 individuals in certain families showed much higherlevels of resistance to SLCV than the original source of resistance,ZGY-46-2604. These F2 individuals were self-pollinated.

F3 families derived from the F2 individuals with unexpectedly highlevels of SLCV resistance were inoculated with a cocktail of ZYMV,WMV-2, and PRSV, and resistant individuals were transplanted to a fieldnear Woodland, Calif. in the summer of 2003. One hundred and two F3individuals were selected for self-pollination from this field based ontheir level of potyvirus resistance, and fruit and plantcharacteristics. Successful pollinations from the California F3generation were sown in Florida to evaluate fruit quality and Sinaloa(for a simultaneous observation under heavy potyvirus and geminivirusnatural pressure), and ten F4 families were selected. The field trial inSinaloa allowed verification of the high level geminivirus resistance inan environment very similar to a commercial field. The selected linesprovided a verification of the unexpected results from the early 2003greenhouse screen, because the F4 generation also showed a higher levelof resistance to natural geminivirus infection than the genotype whichwas the original source of resistance.

Simultaneously with the F3 family testing with ZYMV, WMV-2, and PRSV,these same F3 families were also sent for crosses with elite proprietaryinbred lines, including the cross that eventually led to ZGN-130-1091.When results of the testing from F3 and F4 generation experiments inWoodland and Sinaloa were completed, the TAW F3 families with the bestlevels of resistance for potyvirus and geminivirus had already beencrossed to a battery of elite inbred lines. One of those elite inbredlines was ZGN-130-1020. ZGN-130-1020 was developed from a cross betweenthe Italian landrace known as “Verde Milano Migliorato” and “HMZYR” thatwas distributed by Cornell University through 8 generations ofselection, self pollination, and backcrossing (to “Verde MilanoMigliorato”). The hybrid developed by one elite cross from a TAW F3selection and ZGN-130-1020 was self pollinated in the greenhouse inWoodland Calif. in 2004, and the F2 generation was evaluated in late2004 at Felda, Fla. Individual plants were selected and self-pollinatedbased on phenotype, with the individuals most like a green zucchiniideotype selected (highly productive, short internode length, erectgrowth habit, early maturity, cylindrical immature fruit shape). The F3generation was inoculated with ZYMV, WMV, and SLCV in early 2005, andsurvivors were individually self pollinated in a greenhouse generation.

In the F4 generation no intentional selection pressure was appliedduring a greenhouse generation increase. The F5 generation wasinoculated with a cocktail of ZYMV, WMV, and PRSV, and survivors weregrown in Woodland, Calif. Individual plant selections were based onresistance to viral infection and adherence to the green zucchiniideotype. The F6 generation was planted in the greenhouse at Woodland,Calif. in early 2006, and artificially inoculated with SLCV. The progenyshowed uniform resistance to SLCV, and individual plants were selfpollinated to create the F7. The F7 generation was increased andindividually self pollinated without additional screening in late 2006.In the F8 generation seedlings were artificially inoculated with acocktail of ZYMV, WMV, and PRSV, and were observed to be uniformlyresistant. An individual plant from this F8 generation screen was selfpollinated, and designated as ZGN-130-1091. ZGN-130-1091 derives fromthe progeny of a single F8 generation self-pollinated plant.

The parent lines are uniform and stable, as is a hybrid producedtherefrom. A small percentage of variants can occur within commerciallyacceptable limits for almost any characteristic during the course ofrepeated multiplication. However no variants are expected.

B. PHYSIOLOGICAL AND MORPHOLOGICAL CHARACTERISTICS OF SQUASH HYBRIDSV0201YL, SQUASH LINE LEB-EH-08-877 AND SQUASH LINE ZGN-130-1091

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of squash hybrid SV0201YL and the parent lines thereof.A description of the physiological and morphological characteristics ofsuch plants is presented in Tables 1-3.

TABLE 1 Physiological and Morphological Characteristics of HybridSV0201YL Comparison Variety CHARACTERISTIC SV0201YL Anita 1. SpeciesPepo Pepo 2. Kind/Use squash squash 3. Type summer (vegetable marrow)summer 4. Cotyledon length 39.9 mm 36.9 mm width 27.8 mm 25.5 mm apexrounded tapered veining plainly visible plainly visible color mediumgreen medium green color (RHS Color Chart) 143A 143B Seedling shape ofcotyledons elliptic (Cora, Tivoli) elliptic intensity of green color ofmedium (Cora) medium cotyledons cross section of concave concavecotyledons 5. Mature Plant growth habit bush bush plant type pricklyprickly 6. Main Stem cross-section shape angled angled diameter atmid-point of 24.5 mm 26.1 mm 1^(st) internode average length 43.0 cm 66cm average number of  17.5  20.7 internodes Stem color completely green(Becky) completely green intensity of green color dark (Greyzini) darkmottling absent (Cinderella) present tendrils well developed (Baby Bear,well developed Greyzini) Plant growth habit bush (Greyzini) bushbranching absent (Goldi) absent bush varieties only: semi-erect (Arlesa)semi-erect to horizontal attitude of petiole (excluding lower externalleaves) 7. Leaves blade shape reniform reniform blade form deep lobeddeep lobed margin denticulate denticulate margin edges frilled frilledaverage width 35.2 cm 38.4 cm average length 28.8 cm 29.6 cm leafsurface blistered blistered dorsal surface pubescence soft hairybristled vental surface pubescence soft hairy bristled color dark greendark green color (RHS Color Chart) 147A 147A leaf blotching blotchedwith gray blotched with gray leaf blade: size medium (Ambassador) mediumleaf blade: incisions medium (Jackpot) medium leaf blade: intensity ofdark (Everest) dark green color of upper surface leaf blade: silverypatches present (Civac) present leaf blade: relative area medium(Ambassador) large covered by silvery patches average petiole length34.6 cm 39.7 cm petiole length medium (Goldi) medium petiole: number ofmany (White Bush Scallop) many prickles 8. Flower pistillate flower:average 15.2 cm 13.2 cm diameter pistillate flower: ovary drum-likedrum-like pistillate flower: average 1.2 cm 6.5 cm pedicel lengthpistillate flower: margin curved curved shape pistillate flower: marginfrilled frilled edges pistillate flower: average 1.3 mm 1.3 mm sepalwidth pistillate flower: average 4.6 mm 6.5 mm sepal length pistillateflower: color deep yellow orange orange pistillate flower: color 21A 23A(RHS Color Chart) staminate flower: average 17.6 mm 17.1 mm sepal lengthstaminate flower: average 2.4 mm 2.7 mm sepal width staminate flower:average 165 mm 169.6 mm pedicel length staminate flower: color orangeorange female flower: ring at present (Aurore) present inner side ofcorolla female flower: color of green (Aurore, Early White green ring atinner side of Bush Scallop, President) corolla female flower: intensityof strong (Aristocrat, Diamant) weak green color of ring at inner sideof corolla (varieties with green ring at inner side of corolla) maleflower: ring at inner present (Goldi) present side of corolla maleflower: color of ring green (Austral, Belor, Goldi) yellow and green atinner side of corolla male flower: intensity of strong (Goldi) greencolor of ring at inner side of corolla staminate flower: color 23Aorange 9. Fruit market maturity: average 16.3 cm 16.1 cm length marketmaturity: average 2.8 cm 3.2 cm width - stem end at market maturity: 1.3cm 2.2 cm average width - blossom end market maturity: average 197.4 gm354.6 gm weight market maturity: shape banana butternut according tovariety type market maturity: apex rounded rounded market maturity: baserounded rounded market maturity: ribs none inconspicuous marketmaturity: rib shallow shallow furrow depth market maturity: rib narrownarrow furrow width market maturity: fruit smooth fine wrinkle surfacemarket maturity: warts none none market maturity: blossom raised acornraised acorn scar button young fruit: ratio length/ large (Carlotta)medium maximum diameter (zucchini type varieties) young fruit: generalshape tapered elliptical (Top Kapi) tapered elliptical (zucchini androunded zucchini type varieties) young fruit: main color of green(Elite, Opal, Romano) green skin (excluding color of ribs or grooves)young fruit: intensity of medium (Baccara) light green color of skin(only varieties with green color of skin) general shape club shaped clubshaped length (zucchini type medium (Cora) medium varieties) maximumdiameter small (Goldi) medium (zucchini type varieties) ratiolength/maximum small (Jedida) medium diameter (zucchini type varieties)blossom end (zucchini and rounded rounded neck type varieties) groovespresent absent depth of grooves shallow (Connecticut Field) ribs absentabsent main color of skin green (Ambassador, Baby green (excluding colorof dots, Bear) patches, stripes and bands) intensity of green color ofmedium light skin (only varieties with green color of skin) stripes ingrooves absent (Baby Bear, Jack Be absent Little) dots present (GoldRush, Table present Queen) size of main dots medium (Grey Zucchini) verylarge secondary green color absent (Grey Zucchini, absent between ribs(excluding Small Sugar) dots) warts on skin absent absent size of flowerscar small (Goldi) medium length of peduncle very short (Arlesa) mediumcolor of peduncle green (Ambassador) green intensity of green color ofdark (Gold Rush) medium peduncle mottling of peduncle present (Elite)present ripe fruit: secondary color yellow (Gold Rush) yellow of skin(excluding color of mottles, patches, stripes and bands) ripe fruit:intensity of medium medium main color of skin (only yellow and orange)ripe fruit: color of flesh yellow (Sunburst, Vegetable yellow Spaghetti)ripe fruit: lignified rind present (Elite, Little Gem, presentScallopini, Yellow Summer Crookneck) ripe fruit: structure of fibrous(Vegetable fibrous flesh Spaghetti) 10. Rind average thickness at 1.9 mm2.2 mm medial toughness hard hard overall color pattern regular regularmain or ground color orange-gray yellowish-orange main or ground color163D 18D (RHS Color Chart) color of stripes creamy-yellow creamy-orangeRHS Color Chart for 19B 20C spots color pattern of spots not specificnot specific 11. Flesh average blossom end 35.9 mm 60.3 mm thicknessaverage medial thickness 40.5 mm 55.6 mm average stem end 39.3 mm 44.4mm thickness texture (fine, granular, fine fine lumpy or stringy)texture (soft, firm or soft firm brittle) texture (dry, moist or juicyjuicy juicy) flavor sweet sweet quality excellent good colorcreamy-white creamy-white color (RHS Color Chart) 155A 155B 12. SeedCavity average length 30.7 cm 24.6 cm average width 6.5 cm 7.9 cmlocation conforms to fruit shape conforms to fruit shape placentaltissue abundant moderately abundant center core prominent prominent 13.Fruit Stalks average length 1.4 cm 3.2 cm average diameter 2.4 cm 2.4 cmcross-section shape irregular irregular twisting not twisted not twistedtapering tapered tapered straightness slightly curved slightly curvedtexture hard hard furrows shallow deep surface smooth spiny attachmentend slightly expanded expanded detaches with difficulty with difficultycolor medium green medium green color (RHS Color Chart) 141C 143A 14.Seeds average length 15.8 mm 16.8 mm average width 9.4 mm 9.8 mm averagethickness 2.8 mm 3.3 mm face surface smooth smooth color cream creamcolor (RHS Color Chart) 160D 155A luster dull glossy margin curvedcurved margin edge rounded rounded separation from pulp difficult easyaverage grams per 100 16.0 gm 18.0 gm seeds average number of seeds199.8 138.6 per fruit seed coat normal normal size medium (Diamant)large shape broad elliptic (Baby Boo) elliptic hull present (Baby Bear,Elite) present appearance of hull fully developed (Elite) fullydeveloped color of hull cream (De Nice à Fruit cream Rond) fruit type:zucchini fruit: patches, stripes or absent (Ambassador, Black bands inripe stage (if Jack) zucchini type) *These are typical values. Valuesmay vary due to environment. Other values that are substantiallyequivalent are also within the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of LineZGN-130-1091 Comparison Variety CHARACTERISTIC ZGN 130-1091 Payroll 1.Species Pepo Pepo 2. Kind/Use squash squash 3. Type summer (vegetablemarrow) summer 4. Cotyledon length 39.4 mm 44.27 mm width 29.4 mm 29.13mm apex rounded rounded veining plainly visible plainly visible colormedium green dark green color (RHS Color Chart) 137A 147A Seedling shapeof cotyledons elliptic (Cora, Tivoli) elliptic intensity of green colorof medium (Cora) dark cotyledons cross section of straight (Sunburst)straight cotyledons 5. Mature Plant growth habit bush bush plant typeprickly prickly 6. Main Stem cross-section shape angled round diameterat mid-point of 33 mm 29.79 mm 1^(st) internode average length 35 cm38.6 cm average number of 36 28.56 internodes Stem color completelygreen (Becky) completely green intensity of green color very dark(Goldrush) dark mottling absent (Cinderella) present tendrils absent torudimentary absent to rudimentary (Goldrush, Sylvana) Plant growth habitbush (Greyzini) bush branching present (Patty Green Tint) absent degreeof branching weak (Karioka, Verdi) bush varieties only: semi-erect tohorizontal semi-erect to horizontal attitude of petiole (Goldi)(excluding lower external leaves) 7. Leaves blade shape reniform ovateblade form shallow lobed deep lobed margin denticulate dentate marginedges frilled frilled average width 35 cm 32.92 cm average length 29 cm28.1 cm leaf surface blistered smooth dorsal surface pubescence softhairy bristled vental surface pubescence soft hairy bristled color darkgreen medium green color (RHS Color Chart) 137A 137A leaf blotchingblotched with gray blotched with gray leaf blade: size medium(Ambassador) large leaf blade: incisions medium (Jackpot) medium leafblade: intensity of dark (Everest) medium green color of upper surfaceleaf blade: silvery patches present (Civac) present leaf blade: relativearea medium (Ambassador) medium covered by silvery patches averagepetiole length 32 cm 33.15 cm petiole length medium (Goldi) longpetiole: number of few (Opaline) many prickles 8. Flower pistillateflower: average 11.9 cm 16.39 cm diameter pistillate flower: ovarydrum-like drum-like pistillate flower: average 1.1 cm 2.14 cm pedicellength pistillate flower: margin curved curved shape pistillate flower:margin frilled frilled edges pistillate flower: average 0.9 mm 1.47 mmsepal width pistillate flower: average 3.3 mm 6.14 mm sepal lengthpistillate flower: color orange deep yellow pistillate flower: color 17B17A (RHS Color Chart) staminate flower: average 10.7 mm 19.51 mm sepallength staminate flower: average 1.8 mm 2.75 mm sepal width staminateflower: average 117 mm 162.53 mm pedicel length staminate flower: colororange orange female flower: ring at present (Aurore) present inner sideof corolla female flower: color of green (Aurore, Early White yellow andgreen ring at inner side of Bush Scallop, President) corolla femaleflower: intensity of strong (Aristocrat, Diamant) medium green color ofring at inner side of corolla (varieties with green ring at inner sideof corolla) male flower: ring at inner present (Goldi) present side ofcorolla male flower: color of ring green (Austral, Belor, Goldi) yellowand green at inner side of corolla male flower: intensity of strong(Goldi) medium green color of ring at inner side of corolla staminateflower: color 21A N25D 9. Fruit market maturity: average 19.2 cm 15.7 cmlength market maturity: average 2.5 cm 2.53 cm width - stem end atmarket maturity: 1.1 cm 1.56 cm average width - blossom end marketmaturity: average 221 gm 133.2 gm weight market maturity: shapestraightneck straightneck according to variety type market maturity:apex taper pointed rounded market maturity: base rounded rounded marketmaturity: ribs inconspicuous inconspicuous market maturity: rib shallowshallow furrow depth market maturity: rib medium wide narrow furrowwidth market maturity: fruit smooth smooth surface market maturity:warts none none market maturity: blossom raised acron slightly extendedscar button young fruit: ratio length/ large (Carlotta) large maximumdiameter (zucchini type varieties) young fruit: general shapecylindrical (Ambassador, cylindrical (zucchini and rounded Ibis)zucchini type varieties) young fruit: main color of green (Elite, Opal,Romano) green skin (excluding color of ribs or grooves) young fruit:intensity of very dark (Carnaval, medium green color of skin Corsair)(excluding color of ribs or grooves; only varieties with green color ofskin) general shape cylindrical cylindrical length (zucchini type long(Carlotta) medium varieties) maximum diameter small (Goldi) medium(zucchini type varieties) ratio length/maximum large (Carlotta) largediameter (zucchini type varieties) blossom end (zucchini and pointedrounded neck type varieties) grooves absent present ribs present presentprotrusion of ribs very weak (Leda, Tivoli) weak main color of skingreen (Ambassador, Baby green (excluding color of dots, Bear) patches,stripes and bands) intensity of green color of very dark (Baby Bear,medium skin (only varieties with Sardane) green color of skin) color ofribs compared to same (Grey Zucchini) main color of skin dots absent(Sunburst) absent secondary green color absent (Grey Zucchini, absentbetween ribs (excluding Small Sugar) dots) warts on skin absent absentsize of flower scar medium (Spidi) very small length of peduncle short(Clarita) long color of peduncle green (Ambassador) green intensity ofgreen color of medium (Sunburst) medium peduncle mottling of pedunclepresent (Elite) absent ripe fruit: main color of yellow (Gold Rush)green skin (excluding color of mottles, patches, stripes and bands) ripefruit: intensity of medium main color of skin ripe fruit: secondarycolor green of skin (excluding color of mottles, patches, stripes andbands) ripe fruit: color of flesh cream (Elite) yellow ripe fruit:lignified rind present (Elite, Little Gem, present Scallopini, YellowSummer Crookneck) ripe fruit: structure of fibrous (Vegetable fibrousflesh Spaghetti) 10. Rind average thickness at 1.2 mm 2.03 mm medialtoughness hard hard overall color pattern regular regular main or groundcolor Green greenish gray main or ground color N189A N189A (RHS ColorChart) color of blotches orange color of blotches (RHS 23A Color Chart)pattern of blotches not specific 11. Flesh average blossom end 36.7 mm32.4 mm thickness average medial thickness 40.1 mm 38.1 mm average stemend 41.5 mm 40.1 mm thickness texture (fine, granular, fine stringylumpy or stringy) texture (soft, firm or firm firm brittle) texture(dry, moist or juicy juicy juicy) flavor insipid insipid quality goodgood color whitish cream white color (RHS Color Chart) 8D 155A 12. SeedCavity average length 34.2 cm 34.37 cm average width 6.3 cm 4.92 cmlocation conforms to fruit shape conforms to fruit shape placentaltissue abundant abundant center core inconspicuous prominent 13. FruitStalks average length 2 cm 2.68 cm average diameter 1.8 cm 1.85 cmcross-section shape irregular irregular twisting not twisted not twistedtapering tapered not tapered straightness slightly curved straighttexture hard spongy furrows deep deep surface smooth rough attachmentend expanded slightly expanded detaches easily easily color medium greenmedium green color (RHS Color Chart) 143C 144A 14. Seeds average length14.3 mm 14.1 mm average width 8.4 mm 8.32 mm average thickness 2.4 mm2.42 mm face surface smooth smooth color white cream color (RHS ColorChart) 155A 162D luster glossy dull margin curved curved margin edgerounded rounded separation from pulp moderately easy easy average gramsper 100 11 gm 11.25 gm seeds average number of seeds 193 180.25 perfruit seed coat normal normal size small (Delicata) medium shape broadelliptic (Baby Boo) elliptic hull present (Baby Bear, Elite) presentappearance of hull fully developed (Elite) rudimentary color of hullwhitish (Table Queen) cream For NL Technical Questionnaire: SpecialConditions for the Examination of the Variety fruit type: zucchinifruit: patches, stripes or present (Elite, Greyzini) bands in ripe stage(if zucchini type) *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention.

TABLE 3 Physiological and Morphological Characteristics of LineLEB-EH-08-877 Comparison Variety CHARACTERISTIC LEB-EH-08-877 Anita 1.Species Pepo Pepo 2. Kind/Use squash squash 3. Type summer (vegetablemarrow) summer 4. Cotyledon length 34.6 mm 36.9 mm width 24.0 mm 25.5 mmapex tapered tapered veining plainly visible plainly visible colormedium green medium green color (RHS Color Chart) 143A 143B Seedlingshape of cotyledons narrow elliptic (Bianchini) elliptic intensity ofgreen color of medium (Cora) medium cotyledons cross section of straight(Sunburst) concave cotyledons 5. Mature Plant growth habit bush bushplant type prickly prickly 6. Main Stem cross-section shape round angleddiameter at mid-point of 26.7 mm 26.1 mm 1^(st) internode average length55.1 cm 66 cm average number of  16.4  20.7 internodes Stem colorcompletely green (Becky) completely green intensity of green color light(Bianchini) dark mottling absent (Cinderella) present tendrils absent torudimentary well developed (Goldrush, Sylvania) Plant growth habit bush(Greyzini) bush branching absent (Goldi) absent bush varieties only:semi-erect (Arlesa) semi-erect to horizontal attitude of petiole(excluding lower external leaves) 7. Leaves blade shape reniformreniform blade form deep lobed deep lobed margin dentate denticulatemargin edges frilled frilled average width 36.3 cm 38.4 cm averagelength 26.7 cm 29.6 cm leaf surface blistered blistered dorsal surfacepubescence soft hairy bristled vental surface pubescence soft hairybristled color dark green dark green color (RHS Color Chart) N189A 147Aleaf blotching blotched with gray blotched with gray leaf blade: sizelarge (Kriti) medium leaf blade: incisions shallow (Everest) medium leafblade: intensity of dark (Everest) dark green color of upper surfaceleaf blade: silvery patches present (Civac) present leaf blade: relativearea small (Aziz) large covered by silvery patches average petiolelength 33.4 cm 39.7 cm petiole length long (Autumn Gold, Baikal) mediumpetiole: number of many (White Bush Scallop) many prickles 8. Flowerpistillate flower: average 13.3 cm 13.2 cm diameter pistillate flower:ovary turbinate drum-like pistillate flower: average 1.1 cm 6.5 cmpedicel length pistillate flower: margin curved curved shape pistillateflower: margin frilled frilled edges pistillate flower: average 0.9 mm1.3 mm sepal width pistillate flower: average 8.6 mm 6.5 mm sepal lengthpistillate flower: color deep yellow orange orange pistillate flower:color 21A 23A (RHS Color Chart) staminate flower: average 21.2 mm 17.1mm sepal length staminate flower: average 3.0 mm 2.7 mm sepal widthstaminate flower: average 173.6 mm 169.6 mm pedicel length staminateflower: color deep yellow orange orange female flower: ring at present(Aurore) present inner side of corolla female flower: color of green(Aurore, Early White green ring at inner side of Bush Scallop,President) corolla female flower: intensity of medium (Samba, Senator)weak green color of ring at inner side of corolla (varieties with greenring at inner side of corolla) male flower: ring at inner present(Goldi) present side of corolla male flower: color of ring green(Austral, Belor, Goldi) yellow and green at inner side of corolla maleflower: intensity of strong (Goldi) green color of ring at inner side ofcorolla staminate flower: color 21A orange 9. Fruit market maturity:average 11.3 cm 16.1 cm length market maturity: average 2.9 cm 3.2 cmwidth - stem end at market maturity: 2.1 cm 2.2 cm average width -blossom end market maturity: average 196.3 gm 354.6 gm weight marketmaturity: shape buttercup butternut according to variety type marketmaturity: apex rounded rounded market maturity: base rounded roundedmarket maturity: ribs inconspicuous inconspicuous market maturity: ribshallow shallow furrow depth market maturity: rib medium wide narrowfurrow width market maturity: fruit smooth fine wrinkle surface marketmaturity: warts none none market maturity: blossom raised acorn raisedacorn scar button young fruit: ratio length/ medium (Cora) mediummaximum diameter (zucchini type varieties) young fruit: general shapepear shaped (Clarita) tapered elliptical (zucchini and rounded zucchinitype varieties) young fruit: main color of green (Elite, Opal, Romano)green skin (excluding color of ribs or grooves) young fruit: intensityof light (Arlika) light green color of skin (only varieties with greencolor of skin) general shape pear shaped club shaped length (zucchinitype medium (Cora) medium varieties) maximum diameter medium (Opal)medium (zucchini type varieties) ratio length/maximum medium (Cora)medium diameter (zucchini type varieties) blossom end (zucchini androunded rounded neck type varieties) grooves absent absent ribs absentabsent protrusion of ribs main color of skin green (Ambassador, Babygreen (excluding color of dots, Bear) patches, stripes and bands)intensity of green color of light light skin (only varieties with greencolor of skin) stripes in grooves absent (Baby Bear, Jack Be absentLittle) dots present (Gold Rush, Table present Queen) size of main dotslarge (Kingsize) very large secondary green color absent (Grey Zucchini,absent between ribs (excluding Small Sugar) dots) warts on skin absentabsent size of flower scar medium (Spidi) medium length of peduncleshort (Clarita) medium color of peduncle green (Ambassador) greenintensity of green color of light (Bianchini) medium peduncle mottlingof peduncle present (Elite) present ripe fruit: secondary color whitish(White Bush yellow of skin (excluding color of Scallop) mottles,patches, stripes and bands) ripe fruit: intensity of light medium maincolor of skin (only yellow and orange) secondary color of skin creamcream green hue absent (Jedida) absent ripe fruit: color of flesh cream(Elite) yellow ripe fruit: lignified rind present (Elite, Little Gem,present Scallopini, Yellow Summer Crookneck) ripe fruit: structure offibrous (Vegetable fibrous flesh Spaghetti) 10. Rind average thicknessat 1.7 mm 2.2 mm medial toughness hard hard overall color patternregular regular main or ground color yellow yellowish-orange main orground color 8D 18D (RHS Color Chart) color of stripes creamy-whitecreamy-orange RHS Color Chart for 155A 20C spots color pattern of spotsnot specific not specific 11. Flesh average blossom end 48.8 mm 60.3 mmthickness average medial thickness 51.1 mm 55.6 mm average stem end 45.5mm 44.4 mm thickness texture (fine, granular, fine fine lumpy orstringy) texture (soft, firm or firm firm brittle) texture (dry, moistor juicy juicy juicy) flavor sweet sweet quality excellent good colorcreamy-white creamy-white color (RHS Color Chart) 155A 155B 12. SeedCavity average length 18.1 cm 24.6 cm average width 8.1 cm 7.9 cmlocation conforms to fruit shape conforms to fruit shape placentaltissue abundant moderately abundant center core inconspicuous prominent13. Fruit Stalks average length 2.0 cm 3.2 cm average diameter 2.8 cm2.4 cm cross-section shape irregular irregular twisting not twisted nottwisted tapering not tapered tapered straightness straight slightlycurved texture spongy hard furrows shallow deep surface spiny spinyattachment end slightly expanded expanded detaches with difficulty withdifficulty color light green medium green color (RHS Color Chart) 144B143A 14. Seeds average length 15.6 mm 16.8 mm average width 9.8 mm 9.8mm average thickness 3.4 mm 3.3 mm face surface smooth smooth colorcream cream color (RHS Color Chart) 158B 155A luster glossy glossymargin curved curved margin edge rounded rounded separation from pulpdifficult easy average grams per 100 16.0 gm 18.0 gm seeds averagenumber of seeds 170.4 138.6 per fruit seed coat normal normal sizemedium (Diamant) large shape elliptic (Elite) elliptic hull present(Baby Bear, Elite) present appearance of hull fully developed (Elite)fully developed color of hull cream (De Nice à Fruit cream Rond) fruittype: zucchini fruit: patches, stripes or absent (Ambassador, Blackbands in ripe stage (if Jack) zucchini type) *These are typical values.Values may vary due to environment. Other values that are substantiallyequivalent are also within the scope of the invention.

C. BREEDING SQUASH PLANTS

One aspect of the current invention concerns methods for producing seedof squash hybrid SV0201YL involving crossing squash lines LEB-EH-08-877and ZGN-130-1091. Alternatively, in other embodiments of the invention,hybrid SV0201YL, line LEB-EH-08-877, or line ZGN-130-1091 may be crossedwith itself or with any second plant. Such methods can be used forpropagation of hybrid SV0201YL and/or the squash lines LEB-EH-08-877 andZGN-130-1091, or can be used to produce plants that are derived fromhybrid SV0201YL and/or the squash lines LEB-EH-08-877 and ZGN-130-1091.Plants derived from hybrid SV0201YL and/or the squash linesLEB-EH-08-877 and ZGN-130-1091 may be used, in certain embodiments, forthe development of new squash varieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SV0201YL followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSV0201YL and/or squash lines LEB-EH-08-877 and ZGN-130-1091 for thepurpose of developing novel squash lines, it will typically be preferredto choose those plants which either themselves exhibit one or moreselected desirable characteristics or which exhibit the desiredcharacteristic(s) when in hybrid combination. Examples of desirabletraits may include, in specific embodiments, high seed yield, high seedgermination, seedling vigor, high fruit yield, disease tolerance orresistance, and adaptability for soil and climate conditions.Consumer-driven traits, such as a fruit shape, color, texture, and tasteare other examples of traits that may be incorporated into new lines ofsquash plants developed by this invention.

D. PERFORMANCE CHARACTERISTICS

As described above, hybrid SV0201YL exhibits desirable traits, asconferred by squash lines LEB-EH-08-877 and ZGN-130-1091. Theperformance characteristics of hybrid SV0201YL and squash linesLEB-EH-08-877 and ZGN-130-1091 were the subject of an objective analysisof the performance traits relative to other varieties. The results ofthe analysis are presented below.

TABLE 4 Performance Data of Hybrid SV0201YL and Comparative VarietyESKENDERANY The SV0201YL hybrid was first created in 2009, andevaluations of the hybrid were made in 2010, 2011, and 2012. The resultsfrom the Jordan Valley in early 2011 (low pressure of viral diseases)are typical for performance: Fruit Blossom Marketable Hybrid Fruit ShapeFruit Color Uniformity Scar Ease of Harvest Vigor Growth Habit Spinesfruit/plant SV0201YL 5 3 3 4 4 2 4 5 8.3 SV0201YL 5 4 3 5 5 2 4 4 7.5SV0201YL 5 3 2 5 2 2 3 4 9.2 ESKENDERANY 5 6 2 2 2 3 3 6 9.0 ESKENDERANY5 6 2 3 3 3 3 6 9.8 ESKENDERANY 6 5 3 3 3 4 2 4 7.6

TABLE 5 Performance Data of Hybrid SV0201YL and Comparative VarietyESKENDERANY Also typical for performance under moderate virus pressureare the following results from Los Mochis, Mexico, in 2011: FruitMarketable ASRT3 Fruit Shape Fruit Color Uniformity Blossom Scar VigorGrowth Habit Spines fruit/plant Eskenderany 4 3 6 5 4 4 7 13.0Eskenderany 5 4 5 5 6 2 8 9.9 SV0201YL 3 2 4 6 3 5 6 14.1 SV0201YL 4 2 87 3 4 5 15.5Traits were observed on a sliding scale, where 1 is ideal and 9 isunacceptable.

E. FURTHER EMBODIMENTS OF THE INVENTION

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those squash plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentalsquash plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental squash plant towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a squash plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny squash plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of squash the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiology, 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of squash plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

F. PLANTS DERIVED BY GENETIC ENGINEERING

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Bio-Technology, 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13: 344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985),including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591,1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemlyduplicated version of the CaMV 35S promoter, the enhanced 35S promoter(P-e35S); 1 the nopaline synthase promoter (An et al., Plant Physiol.,88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell,1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as describedin U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter(P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem;the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform viruspromoter; a commelina yellow mottle virus promoter; and other plant DNAvirus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., PlantPhysiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter,Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter,Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-bindingprotein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones,such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4)wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5)chemicals such as methyl jasmonate, salicylic acid, or Safener. It mayalso be advantageous to employ organ-specific promoters (e.g., Roshal etal., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a squash plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a squash plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

G. DEFINITIONS

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) color chart value: The RHS color chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightnessand saturation. A color is precisely named by the RHS color chart byidentifying the group name, sheet number and letter, e.g., Yellow-OrangeGroup 19A or Red Group 41B.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a squashvariety are recovered in addition to the characteristics of the singlelocus transferred into the variety via the backcrossing technique and/orby genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a squash plant by transformation.

H. DEPOSIT INFORMATION

A deposit of squash hybrid SV0201YL and inbred parent linesLEB-EH-08-877 and ZGN-130-1091, disclosed above and recited in theclaims, has been made with the American Type Culture Collection (ATCC),10801 University Blvd., Manassas, Va. 20110-2209. The date of depositwere Nov. 20, 2012, Nov. 4, 2013, and Nov. 20, 2012, respectively. Theaccession numbers for those deposited seeds of squash hybrid SV0201YLand inbred parent lines LEB-EH-08-877 and ZGN-130-1091 are ATCCAccession No. PTA-13335, ATCC Accession No. PTA-120694, and ATCCAccession No. PTA-13334, respectively. Upon issuance of a patent, allrestrictions upon the deposits will be removed, and the deposits areintended to meet all of the requirements of 37 C.F.R. §1.801-1.809. Thedeposits will be maintained in the depository for a period of 30 years,or 5 years after the last request, or for the effective life of thepatent, whichever is longer, and will be replaced if necessary duringthat period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed is:
 1. A squash plant comprising at least a first set ofthe chromosomes of squash line LEB-EH-08-877 or squash lineZGN-130-1091, a sample of seed of said lines having been deposited underATCC Accession Number PTA-120694 and ATCC Accession Number PTA-13334,respectively.
 2. A seed comprising at least a first set of thechromosomes of squash line LEB-EH-08-877 or squash line ZGN-130-1091, asample of seed of said lines having been deposited under ATCC AccessionNumber PTA-120694 and ATCC Accession Number PTA-13334, respectively. 3.The plant of claim 1, which is inbred.
 4. The plant of claim 1, which ishybrid.
 5. The seed of claim 2, which is inbred.
 6. The seed of claim 2,which is hybrid.
 7. The plant of claim 4, wherein the hybrid plant issquash hybrid SV0201YL, a sample of seed of said hybrid SV0201YL havingbeen deposited under ATCC Accession Number PTA-13335.
 8. The seed ofclaim 6, defined as a seed of squash hybrid SV0201YL, a sample of seedof said hybrid SV0201YL having been deposited under ATCC AccessionNumber PTA-13335.
 9. The seed of claim 2, defined as a seed of lineLEB-EH-08-877 or line ZGN-130-1091.
 10. A plant part of the plant ofclaim
 1. 11. The plant part of claim 10, further defined as a leaf, anovule, pollen, a fruit, or a cell.
 12. A squash plant having all thephysiological and morphological characteristics of the squash plant ofclaim
 7. 13. A tissue culture of regenerable cells of the plant ofclaim
 1. 14. The tissue culture according to claim 13, comprising cellsor protoplasts from a plant part selected from the group consisting ofembryos, meristems, cotyledons, pollen, leaves, anthers, roots, roottips, pistil, flower, seed and stalks.
 15. A squash plant regeneratedfrom the tissue culture of claim
 13. 16. A method of vegetativelypropagating the plant of claim 1 comprising the steps of: (a) collectingtissue capable of being propagated from a plant according to claim 1;(b) cultivating said tissue to obtain proliferated shoots; and (c)rooting said proliferated shoots to obtain rooted plantlets.
 17. Themethod of claim 16, further comprising growing at least a first plantfrom said rooted plantlets.
 18. A method of introducing a desired traitinto a squash line comprising: (a) crossing a plant of lineLEB-EH-08-877 or ZGN-130-1091 with a second squash plant that comprisesa desired trait to produce F1 progeny, a sample of seed of said lineshaving been deposited under ATCC Accession Number PTA-120694, and ATCCAccession Number PTA-13334, respectively; (b) selecting an F1 progenythat comprises the desired trait; (c) backcrossing the selected F1progeny with a plant of line LEB-EH-08-877 or ZGN-130-1091 to producebackcross progeny; (d) selecting backcross progeny comprising thedesired trait and the physiological and morphological characteristic ofsquash line LEB-EH-08-877 or ZGN-130-1091; and (e) repeating steps (c)and (d) three or more times to produce selected fourth or higherbackcross progeny that comprise the desired trait.
 19. A squash plantproduced by the method of claim
 18. 20. A method of producing a plantcomprising an added trait, the method comprising introducing a transgeneconferring the trait into a plant of hybrid SV0201YL, line LEB-EH-08-877or line ZGN-130-1091, a sample of seed of said hybrid and lines havingbeen deposited under ATCC Accession Number PTA-13335, ATCC AccessionNumber PTA-120694, and ATCC Accession Number PTA-13334, respectively.21. A plant produced by the method of claim
 20. 22. The plant of claim1, comprising a transgene.
 23. The plant of claim 22, wherein thetransgene confers a trait selected from the group consisting of malesterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, modified fatty acid metabolism, environmental stresstolerance, modified carbohydrate metabolism and modified proteinmetabolism.
 24. The plant of claim 1, comprising a single locusconversion.
 25. The plant of claim 24, wherein the single locusconversion confers a trait selected from the group consisting of malesterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, modified fatty acid metabolism, environmental stresstolerance, modified carbohydrate metabolism and modified proteinmetabolism.
 26. A method for producing a seed of a plant derived from atleast one of hybrid SV0201YL, line LEB-EH-08-877 or line ZGN-130-1091comprising the steps of: (a) crossing a squash plant of hybrid SV0201YL,line LEB-EH-08-877 or line ZGN-130-1091 with itself or a second squashplant; a sample of seed of said hybrid and lines having been depositedunder ATCC Accession Number PTA-13335, ATCC Accession Number PTA-120694,and ATCC Accession Number PTA-13334, respectively; and (b) allowing seedof a hybrid SV0201YL, line LEB-EH-08-877 or line ZGN-130-1091-derivedsquash plant to form.
 27. The method of claim 26, further comprising thesteps of: (c) selfing a plant grown from said hybrid SV0201YL,LEB-EH-08-877 or ZGN-130-1091-derived squash seed to yield additionalhybrid SV0201YL, line LEB-EH-08-877 or line ZGN-130-1091-derived squashseed; (d) growing said additional hybrid SV0201YL, line LEB-EH-08-877 orline ZGN-130-1091-derived squash seed of step (c) to yield additionalhybrid SV0201YL, line LEB-EH-08-877 or line ZGN-130-1091-derived squashplants; and (e) repeating the crossing and growing steps of (c) and (d)to generate at least a first further hybrid SV0201YL, line LEB-EH-08-877or line ZGN-130-1091-derived squash plant.
 28. The method of claim 26,wherein the second squash plant is of an inbred squash line.
 29. Themethod of claim 26, comprising crossing line LEB-EH-08-877 with lineZGN-130-1091, a sample of seed of said lines having been deposited underATCC Accession Number PTA-120694, and ATCC Accession Number PTA-13334,respectively.
 30. The method of claim 27, further comprising: (f)crossing the further hybrid SV0201YL, LEB-EH-08-877 orZGN-130-1091-derived squash plant with a second squash plant to produceseed of a hybrid progeny plant.
 31. A plant part of the plant of claim7.
 32. The plant part of claim 31, further defined as a leaf, a flower,a fruit, an ovule, pollen, or a cell.
 33. A method of producing a squashseed comprising crossing the plant of claim 1 with itself or a secondsquash plant and allowing seed to form.
 34. A method of producing asquash fruit comprising: (a) obtaining a plant according to claim 1,wherein the plant has been cultivated to maturity; and (b) collecting asquash from the plant.